ÇѱÛÁ¦¸ñ(Korean Title) |
ÀçÁ¶ÇÕ º£Å§·Î¹ÙÀÌ·¯½º º¤ÅÍÀÇ Á¦Á¶¿Í °¨¿° |
¿µ¹®Á¦¸ñ(English Title) |
Construction and Transfection of Recombinant Baculovirus Vectors |
ÀúÀÚ(Author) |
»ç¿µÈñ
À̱âȯ
È«¼º°©
Young Hee Sa
Ki Hwan Lee
Seong-Karp Hong
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¿ø¹®¼ö·Ïó(Citation) |
VOL 21 NO. 01 PP. 0700 ~ 0703 (2017. 06) |
Çѱ۳»¿ë (Korean Abstract) |
º£Å§·Î¹ÙÀÌ·¯½º º¤ÅÍ°¡ uroplakin II promoter, polyhedron promoter, vesicular stomatitis virus G(VSVG), enhanced green fluorescent protein (EGFP), protein transduction domain (PTD) µîÀÇ À¯ÀüÀÚ·Î ÀçÁ¶ÇÕ µÇ¾ú´Ù. ÀÌ·¸°Ô ÀçÁ¶ÇÕµÈ º£Å§·Î¹ÙÀÌ·¯½º º¤Å͵éÀº ¿©·¯°¡Áö ¼¼Æ÷ÁÖ¿¡ °¨¿°À» ½ÃÄ×´Ù. ¿ì¸®´Â ÀÌ ÀçÁ¶ÇÕ º¤ÅÍ¿Í ´Ù¸¥ ´ëÁ¶ º¤Å͸¦ ºñ±³ÇÏ¿© À¯ÀüÀÚ Àü´Þ°ú À¯ÀüÀÚ ¹ßÇöÀ» ºñ±³ÇÏ¿´´Ù. ±× °á°ú ÀÌ ÀçÁ¶ÇÕ º£Å§·Î¹ÙÀÌ·¯½º º¤ÅÍ´Â ´ëÁ¶ º¤ÅÍ¿¡ ºñÇÏ¿© À¯ÀüÀÚ Àü´Þ°ú À¯ÀüÀÚ ¹ßÇöÀÇ È¿À²ÀÌ ¿ì¼öÇÑ °ÍÀ¸·Î ³ªÅ¸³µ´Ù.
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¿µ¹®³»¿ë (English Abstract) |
Baculovirus vectors were recombined using uroplakin II promoter, polyhedron promoter, vesicular stomatitis virus G (VSVG), enhanced green fluorescent protein (EGFP), protein transduction domain (PTD) gene and so on. These novel recombinant vectors were infected into various cell lines. We performed and analyzed gene transfer and gene expression of these recombinant vectors comparison with other control vectors. From this result, we identified that these recombinant vectors have higher efficient gene transfer and expression of than control vector.
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Å°¿öµå(Keyword) |
baculovirus
protein transduction domain
uroplakin II promoter
vesicular stomatitis virus G
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ÆÄÀÏ÷ºÎ |
PDF ´Ù¿î·Îµå
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