ÇѱÛÁ¦¸ñ(Korean Title) |
ÀçÁ¶¼ºµÈ º£Å§·Î¹ÙÀÌ·¯½º º¤ÅÍÀÇ À¯ÀüÀÚ ÀüÀÌ¿Í ¹ßÇö |
¿µ¹®Á¦¸ñ(English Title) |
Gene Transfer and Expression of Newly Reconstructed Baculovirus Vectors |
ÀúÀÚ(Author) |
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Ji-Young Kim
Hyun Joo Kim
Young-Hee Sa
Seong-Karp Hong
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¿ø¹®¼ö·Ïó(Citation) |
VOL 20 NO. 02 PP. 0923 ~ 0926 (2016. 10) |
Çѱ۳»¿ë (Korean Abstract) |
º£Å§·Î¹ÙÀÌ·¯½º º¤ÅÍ°¡ cytomegalovirus (CMV) promoter, polyhedron promoter, vesicular stomatitis virus G (VSVG), enhanced green fluorescent protein (EGFP), protein transduction domain (PTD)ÀÇ À¯ÀüÀÚ·Î ÀçÁ¶¼º µÇ¾ú´Ù. ÀÌ·¸°Ô ÀçÁ¶¼ºµÈ º£Å§·Î¹ÙÀÌ·¯½º º¤ÅÍ´Â ´Ù¾çÇÑ ¼¼Æ÷ÁÖ¿Í Á¶Á÷¿¡ °¨¿°½ÃÄ×´Ù. ¿ì¸®´Â ÀÌ ÀçÁ¶¼ºµÈ º¤ÅÍ¿Í ´Ù¸¥ ´ëÁ¶ º¤Å͸¦ ºñ±³ÇÏ¿© À¯ÀüÀÚÀÇ ÀüÀÌ¿Í À¯ÀüÀÚ ¹ßÇöÀ» ºñ±³ÇÏ¿´´Ù. °á·ÐÀûÀ¸·Î ÀÌ ÀçÁ¶¼ºµÈ º£Å§·Î¹ÙÀÌ·¯½º º¤ÅÍÀÇ À¯ÀüÀÚÀÇ ÀüÀÌ¿Í ¹ßÇöÀÇ È¿À²ÀÌ ´ëÁ¶ º¤ÅÍ º¸´Ù ¿ì¼öÇÑ È¿À²À» ³ªÅ¸³»¾ú´Ù.
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¿µ¹®³»¿ë (English Abstract) |
Baculovirus vectors were reconstructed using cytomegalovirus (CMV) promoter, polyhedron promoter, vesicular stomatitis virus G (VSVG), enhanced green fluorescent protein (EGFP), and protein transduction domain (PTD) genes. These reconstructed vector was transfected into various cell lines and tissues. We compared this reconstructed vector with other control vectors in view of gene transfer and gene expression. In conclusion, we confirmed that gene transfer and expression of these reconstructed vectors was higher efficient than any other control vector.
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Å°¿öµå(Keyword) |
baculovirus
enhanced green fluorescent protein
protein transduction domain
reconstructed
vesicular stomatitis virus G
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ÆÄÀÏ÷ºÎ |
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