JIPS (한국정보처리학회)
Current Result Document : 2 / 2
| 한글제목(Korean Title) |
A Study on an Automatic Multi-Focus System for Cell Observation |
| 영문제목(English Title) |
A Study on an Automatic Multi-Focus System for Cell Observation |
| 저자(Author) |
Jaeyoung Park
Sangjoon Lee
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| 원문수록처(Citation) |
VOL 15 NO. 01 PP. 0047 ~ 0054 (2019. 02) |
한글내용
(Korean Abstract) |
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영문내용
(English Abstract) |
This study is concerned with the mechanism and structure of an optical microscope and an automatic multifocus algorithm for automatically selecting sharp images from multiple foci of a cell. To obtain precise cell images quickly, a z-axis actuator with a resolution of 0.1 μm was designed to control an optical microscope Moreover, a lighting control system was constructed to select the color and brightness of light that best suit the object being viewed. Cell images are captured by the instrument and the sharpness of each image is determined using Gaussian and Laplacian filters. Next, cubic spline interpolation and peak detection algorithms are applied to automatically find the most vivid points among multiple images of a single object. A cancer cell imaging experiment using propidium iodide staining confirmed that a sharp multipoint image can be obtained using this microscope. The proposed system is expected to save time and effort required to extract suitable cell images and increase the convenience of cell analysis.
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| 키워드(Keyword) |
Automatic Multi-Focus
Cell Observation
Cubic Spline Interpolation
Peak Detection
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| 파일첨부 |
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